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eos3 2 sequence  (Addgene inc)


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    Addgene inc eos3 2 sequence
    Eos3 2 Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eos3 2 sequence/product/Addgene inc
    Average 92 stars, based on 8 article reviews
    eos3 2 sequence - by Bioz Stars, 2026-02
    92/100 stars

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    Image Search Results


    bPGCLC induction. (A) Scheme for the direct induction of bPGCLCs from bES cells. (B) PGCLC induction with a WNT agonist plus WNT antagonist. Shown are bright-field (BF), fluorescence images and FACS analysis of D4 aggregates of bES cells. BT, BLIMP1-tdTomato. TN, TFAP2C-mNeonGreen. Scale bar: 200 µm. (C) Scheme for the induction of bPGCLCs under the culture conditions indicated. (D) bES cells after 24 h of incubation under the conditions indicated. (E) PGCLC induction with BMP4 and a WNT antagonist. Shown are bright-field (BF) and fluorescence images and FACS patterns of D4 aggregates of bES cells after 24 h of pre-incubation. BT, BLIMP1-tdTomato. TN, TFAP2C-mNeonGreen. Scale bar: 200 µm. (F) PGCLC induction from various cell lines. C1-144 yielded the most BTTN-positive cells when the BMP4 concentration was 200 ng/ml, while C11-928 and N5-319 were best when the BMP4 concentration was 50 ng/ml. The concentration of CHIR was 6 µM in the all experiments. FACS analyses were done on day 4 of PGCLC induction. BT, BLIMP1-tdTomato. TN, TFAP2C-mNeonGreen. Scale bar: 200 µm.

    Journal: The Journal of Reproduction and Development

    Article Title: Efficient derivation of embryonic stem cells and primordial germ cell-like cells in cattle

    doi: 10.1262/jrd.2023-087

    Figure Lengend Snippet: bPGCLC induction. (A) Scheme for the direct induction of bPGCLCs from bES cells. (B) PGCLC induction with a WNT agonist plus WNT antagonist. Shown are bright-field (BF), fluorescence images and FACS analysis of D4 aggregates of bES cells. BT, BLIMP1-tdTomato. TN, TFAP2C-mNeonGreen. Scale bar: 200 µm. (C) Scheme for the induction of bPGCLCs under the culture conditions indicated. (D) bES cells after 24 h of incubation under the conditions indicated. (E) PGCLC induction with BMP4 and a WNT antagonist. Shown are bright-field (BF) and fluorescence images and FACS patterns of D4 aggregates of bES cells after 24 h of pre-incubation. BT, BLIMP1-tdTomato. TN, TFAP2C-mNeonGreen. Scale bar: 200 µm. (F) PGCLC induction from various cell lines. C1-144 yielded the most BTTN-positive cells when the BMP4 concentration was 200 ng/ml, while C11-928 and N5-319 were best when the BMP4 concentration was 50 ng/ml. The concentration of CHIR was 6 µM in the all experiments. FACS analyses were done on day 4 of PGCLC induction. BT, BLIMP1-tdTomato. TN, TFAP2C-mNeonGreen. Scale bar: 200 µm.

    Article Snippet: Existing targeting vectors were treated with restriction enzymes, and mNeonGreen sequences were amplified by PCR from LifeAct-mNeonGreen (98877; Addgene) and cloned using an in-fusion HD cloning kit (TaKaRa Bio, Shiga, Japan).

    Techniques: Fluorescence, Incubation, Concentration Assay

    Separation of bPGCLCs by surface markers. (A) FACS analysis of surface marker protein expression in BTTN-positive and -negative cells in D6 aggregate. Red and grey histograms show the cells with antibody and without antibody, respectively. BT, BLIMP1-tdTomato. TN, TFAP2C-mNeonGreen. (B) Separation of induced PGCLCs (C1-144 ES cell) by surface markers KIT/CD117 and CD44. BT, BLIMP1-tdTomato. TN, TFAP2C-mNeonGreen. (C) Separation of PGCLCs derived from non-reporter bES cells by surface markers. (D) Comparison of gene expression in BTTN+ cells and KIT+/CD44– cells. Shown are ΔC t values determined by qPCR analysis.

    Journal: The Journal of Reproduction and Development

    Article Title: Efficient derivation of embryonic stem cells and primordial germ cell-like cells in cattle

    doi: 10.1262/jrd.2023-087

    Figure Lengend Snippet: Separation of bPGCLCs by surface markers. (A) FACS analysis of surface marker protein expression in BTTN-positive and -negative cells in D6 aggregate. Red and grey histograms show the cells with antibody and without antibody, respectively. BT, BLIMP1-tdTomato. TN, TFAP2C-mNeonGreen. (B) Separation of induced PGCLCs (C1-144 ES cell) by surface markers KIT/CD117 and CD44. BT, BLIMP1-tdTomato. TN, TFAP2C-mNeonGreen. (C) Separation of PGCLCs derived from non-reporter bES cells by surface markers. (D) Comparison of gene expression in BTTN+ cells and KIT+/CD44– cells. Shown are ΔC t values determined by qPCR analysis.

    Article Snippet: Existing targeting vectors were treated with restriction enzymes, and mNeonGreen sequences were amplified by PCR from LifeAct-mNeonGreen (98877; Addgene) and cloned using an in-fusion HD cloning kit (TaKaRa Bio, Shiga, Japan).

    Techniques: Marker, Expressing, Derivative Assay, Comparison, Gene Expression